Nitrogen sources: peptone, protein powder, amino acids, protein peptides, yeast powder, nitrate and so on. Different culture organisms use different nitrogen sources. There are organic and inorganic nitrogen sources that need to be distinguished in particular.
Carbon sources: Monosaccharides, polysaccharides, starches, sucrose, etc. Agar can also provide part of the carbon source for the culture medium, so early agar with more.
Vitamins: The need to provide a variety of individual microbial culture, a wide range, basically the same types of nutrients and nutrition, not one by one here.
Minerals: also known as trace elements, iron, manganese, zinc, selenium, and so on.
PH regulators: including dilute hydrochloric acid and sodium carbonate, respectively, to adjust the PH value of the role of two directions.
Buffer additives: solid medium including the use of thickener, some medium need to do saline buffer and so on, especially animal tissue culture, for medium buffer requirements.
Other ingredients: such as serum, Haemophilus culture must be added to the serum can, the other a variety of different microorganisms have their own unique needs.